Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1
نویسندگان
چکیده
The mushroom cell wall contains polysaccharides that can activate cells of the innate immune system through receptors such as Toll-like (TLR) and dectin-1. In present study, Pleurotus eryngii polysaccharide fractions containing a 3-O methylated mannogalactan (1?3)/(1?6)-?-d-glucans were isolated extensively characterized by 2D NMR methylation analysis. Traces (1?3)-?-d-glucan (1?2)-?-d-mannan also observed. Affinity for TLR2, TLR2-TLR6 dectin-1 using HEK-cells expressing relevant receptor genes was tested. PeWN, mannogalactan, inactive towards whereas fraction PeWB, more ?-glucan, activated heterodimer. Activation human ?-glucan correlated with amount in each fraction. Nitric oxide cytokine supernatant levels D2SC/1 dendritic stimulated P. interferon-? low to moderate. results indicate immunomodulatory activity water-soluble is modest.
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ژورنال
عنوان ژورنال: Carbohydrate Polymers
سال: 2021
ISSN: ['1879-1344', '0144-8617']
DOI: https://doi.org/10.1016/j.carbpol.2021.117991